Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Apoptosis Inhibitor of Macrophages Contributes to the Chronicity of Mycobacterium avium Infection by Promoting Foamy Macrophage Formation.

doi: 10.4049/jimmunol.2200306

Figure Lengend Snippet: FIGURE 6. Histopathological characteristics and accumulation of FMs in recombinant AIM-treated mice after M. avium infection. (A) Based on the total lung cell count, the absolute numbers of AMs and IMs were calculated according to Fig. 2B. Bars, mean ± SD (n 5 34/group). Results were confirmed using three independent experiments. (B) MFI of Pacific Blue in FMs from mice at 4 wk after infection. Bars, mean ± SD (n 5 34/group). Results were confirmed using three independent experiments. (C) Representative images of H&E staining of mouse lungs 4 wk after infection. Upper image, low-power field (40×); lower image, high-power field (400×). Scale bars, 200 and 20 mm in low- and high-power fields, respectively. (D) Representative images of Oil Red O staining of mouse lungs 4 wk after infection. Upper image, low-power field (100×); lower image, high-power field (400×). Scale bars, 100 and 20 mm in low- and high-power fields, respectively. Results were con- firmed using two independent experiments. (E) For quantification of Oil Red Opositive cells in the lungs, positive areas were evaluated with ImageJ Fiji software at ×400 magnification. Assessments were made in at least six randomly selected separate regions for each mouse. Bars, mean ± SD (n 5 3/group). (F) mRNA levels of Nceh1, Abca1, Abcg1, and Acat1 in the lungs at 4 wk after infection. Data are expressed as fold increase. Bars, mean ± SD (n 5 34/group). Results were confirmed using three independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: In some experiments, 1 mg recombinant AIM (2834-CL; R&D Systems, Minneapolis, MN) was administered intranasally or i.v. to mice once per week starting on the day of infection.

Techniques: Recombinant, Infection, Cell Counting, Staining, Software

Journal: Nature Communications

Article Title: Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

doi: 10.1038/s41467-023-39693-x

Figure Lengend Snippet: a Plasma CD5L concentration of CAA patients ( N = 12) and HC ( N = 12) as well as CAA models ( N = 5 in each group) and WT C57/BL6 mice ( N = 5). Data are presented as mean values ± SEM with the indicated significance (by two-tailed Student’s t test). b Left: CD5L deposition in mice brain was evaluated with immunostaining. Right: Immunostaining of CD31 (red), CD5L (green), C5b-9 (blue) and TSPAN4 (purple). Data are representative of 4 biologically independent experiments. c Plasma C5-9 concentration of mice. N = 6 in PBS-M, Aβ40-M and CD5L KO Aβ40-M transferred mice and N = 5 in CD5L KO PBS-M treated group. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). d C5b-9 deposition in brain as assessed with immunostaining. Data are representative of 3 biologically independent experiments. e Fluorescent intensity of 3kDa-Dextran in the plasma and eyeballs of the recipients. N = 6 in PBS-M, Aβ40-M and CD5L KO Aβ40-M transferred mice and N = 5 in CD5L KO PBS-M treated group. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). f Leakage of 3kDa-Dextran in brain parenchyma was assessed with fluorescent microscopy. Data are representative of 3 biologically independent experiments. g Coronal brain sections of the recipients were subjected to immunostaining of CD31 (green) and ZO-1 (red). Data are representative of 3 biologically independent experiments. h Skin autopsy sample of a CAA patient (Left) and brain tissue of WT and Tg-SwDI/B +/+ mice (24w of age) (Right) were subjected to IEM with CD5L staining. Blue dash lines outline micro-vessel wall. Red stars emphasize blood vessel lumen. Red arrow heads emphasize peri-vessel macrophages. Red arrows emphasize CD5L deposition in blood vessels within migrasome-like structures. Data are representative of 3 biologically independent experiments. Source data are provided as a Source Data file.

Article Snippet: Concentration of mouse NEFL (FineTest, EM1688), mouse C5b-9 (FineTest, EM1392), mouse CD5L (Boster, EK1414), human CD5L (MEIMIAN, MM-50587H1), human Aβ40 (CUSABIO, CSB-E08299h), human Aβ42 (MAI Bio, LM-2685H), and human C5b-9 (FineTest, EH3858) in plasma was assessed with commercial kits according to instructions of manufacturers.

Techniques: Clinical Proteomics, Concentration Assay, Two Tailed Test, Immunostaining, Microscopy, Staining